陈同生个人主页
招收物理学、光学工程、生物学和化学专业的硕士和博士研究生以及博士后，欢迎联系（chentsh@scnu.edu.cn; 18588869936）
简介：陈同生，1964年3月出生，二级教授，博士生导师，华南师范大学信息光电子科技学院院长、生物光子学研究院院长，广东省激光生命科学重点实验室主任，华南师范大学高层次人才特殊计划领军人才，生物光子学学科带头人，广东省“千百十人才工程”省级培养对象。广东省生物医学工程学会副理事长，中国光学学会生物医学光子学学会常务理事，中国生物医学工程学会生物医学光子学学会常务理事，中国光学工程学会激光技术及应用专家委员会委员（激光医学），激光生物学报常务编委。主持国家重大科研仪器研制项目、国家基金委重点项目、国家863项目和国家自然科学基金项目等国家级科研项目8项，主持广东省重点领域研发计划、广东省科技计划项目、广东省高教厅重点项目和广东省自然科学基金项目等省部级科研项目10余项，研究总经费超4000万元。研究方向包括显微成像技术及其在生物医学中的应用。
研究方向：
(1)生物医学光子学；
(2)荧光共振能量转移(FRET)显微荧光成像技术及其仪器研发；
(3)细胞生物学：细胞信号转导分子机制的研究。
教育简历：
1982.9-1986.6：湖北师范学院物理专业毕业，获理学学士学位；
1990.9-1993.5：华中科技大学光电子与物理电子专业毕业，获理学硕士学位；
1999.9-2003.5：华中科技大学生物医学工程专业毕业，获工学博士学位；
工作简历：
1986.6-1990.9：湖北师范学院物理系教师，助教；
1993.9-2000.9：湖北师范学院物理系教师，讲师；
2000.9-2003.11：华中科技大学生命科学院，副教授；
2003.11-2005.12：华南师范大学，副教授, 副所长；
2005.12-2009.10 华南师范大学激光生命科学教育部重点实验室 教授，博士导师
2009.10-2018.06 华南师范大学生物光子学研究院&激光生命科学教育部重点实验室 教授，博士导师，学术委员会主任。
2018.07-2020.7 华南师范大学生物光子学研究院&激光生命科学教育部重点实验室 教授，博士导师，院长，实验室主任
2020.07-2021.06 华南师范大学生物光子学研究院&激光生命科学教育部重点实验室 教授，博士导师，院长，实验室主任
2021.06-至今 华南师范大学生物光子学研究院&激光生命科学教育部重点实验室 教授，博士导师，院长，实验室主任；华南师范大学-松山湖材料学实验室 材料科学与工程联合实验中心 主任
近期新闻报道记录
1 陈同生教授参加全省高质量发展大会并接受采访
南方网报道（视频）1'20"~1'45"
2024-2-18
https://xapp.southcn.com/node_2ea31fe5fd/257adee53c.shtml
2 大咖谈｜陈同生：现代化产业体系建设须打好底层工业核心基础
南方都市报
2024-02-17
https://m.mp.oeeee.com/a/BAAFRD000020240217911065.html?layer=2&share=chat&isndappinstalled=0
3 华师新鲜血液！+360！
2023年新教师入职，陈同生教授代表教师代表发言。
晚安华师公众号
2023-09-14
https://mp.weixin.qq.com/s/m9o4-hQnsuN9UEZvBw3xHQ
4 华师新闻网生物光子学研究院罗泽伟博士团队获得第一届粤港澳大湾区博士博士后创新创业大赛金奖
华师新闻网
2023-03-07
https://news.scnu.edu.cn/56551
5 华师金奖！技术领跑全球！
晚安华师公众号
2023-03-06
https://mp.weixin.qq.com/s/a9tHyE_r3riy8LqCr5M1_A
6 生物光子学研究院陈同生教授团队获批广东省重点领域研发计划项目
华师新闻网
2022-06-20
https://news.scnu.edu.cn/51735
7 晚安华师公众号（视频）再见啦华师！我们毕业了！
2022年毕业典礼，陈同生教授作为教师代表发言《相信选择，展翅翱翔》
晚安华师公众号（视频）
2022-06-27
https://mp.weixin.qq.com/s/64fhSqTIEr4rQ2h_zGsO6g
8 公布！这些导师和团队，华师学生最喜爱！
陈同生教授获得“我最喜爱的导师”
晚安华师公众号
2022-06-09
https://mp.weixin.qq.com/s/96ahdZXwv2YEe0zixTRD3Q
9 华师新闻网生物光子学研究院陈同生教授团队荣获第一届全国博士后创新创业大赛金奖
华师新闻网
2021-12-21
https://news.scnu.edu.cn/48094
10 博士后陈同生：专注生物显微成像技术二十载 实现领跑世界
佛山新闻（视频）
2021-10-20
https://www.bilibili.com/video/BV1XF411e7TC
11 生物光子学研究院陈同生教授团队获得2021年广东“众创杯”创业创新大赛博士博士后创新赛团队组金奖
华师新闻网
2021-09-06
https://news.scnu.edu.cn/43956
主持承担的主要基金项目
1. 广东省重点领域研发计划“精密仪器设备”专题项目（主持）:“荧光-FRET-无标记相位三模态活细胞超分辨成像系统的研制”（Guangdong Provincial key research and development program，项目编号：2022B0303040003），立项总经费2000万元(省财政经费1000万元，配套经费1000万元)。执行时间：2022.06-2025.05
2. 国家自然科学基金重点项目（主持）：项目名称：基于结构光照明的动态超分辨定量FRET显微成像研究(62135003)，直接经费306万，起止年月：2022.01-2026.12
3. 国家自然科学基金面上项目（主持）：项目名称：活细胞FRET双杂交检测技术及其应用研究(61875056);直接经费：63万元；起止年月：2019.01-2022.12
4. 国家重大科研仪器研制项目（主持）：定量FRET显微成像仪的研制(61527825)，研究总经费510.32万元，起止年月：2016.01-2020.12
5. 国家自然科学基金面上项目（主持）：基于线性光谱分离的活细胞实时定量FRET显微成像技术研究(81471699) 80万，起止年月：2015.01-2018.12
6. 广东省科技计划项目（主持）：活细胞FRET光谱定量检测仪的研制(2014B090901060)， 100万, 起止年月：2015.01-2018.12
7. 广州市科技计划项目（主持）：光谱FRET定量检测分析技术研究（2014J4100055）, 8万元，起止年月：2014.04-2016.04
8. 国家自然科学基金面上项目（主持）：活细胞中蛋白质寡聚化动态过程实时荧光成像与定量分析技术研究(61178078)， 78万元，起止年月：2012.1-2015.12.
9. 国家自然科学基金面上项目（主持）：显微光学成像术研究紫杉醇诱导肿瘤细胞内质网空泡化和类副凋亡的分子调控机理 (31071218)， 35万元，起止年月：2011.1-2013.12.
10. 广东省高教厅、财政厅重点项目（主持）：非小细胞肺癌对吉非替尼耐药分子机制的单细胞可视化研究 (cxzd115)，25万元，起止年月：2011.10-2014.10。
11. 国家自然科学基金面上项目（主持）：荧光技术在活细胞中实时研究中药诱导肿瘤细胞凋亡的分子机理(30670507)，28万元，起止年月：2007.1-2009.12。
专利技术
(1)陈同生，曾绍群，骆清铭. 基于调Q脉冲激光的多光子激发荧光方法. 国家发明专利，2004，专利号：ZL 01106653.9
(2)曾绍群，陈同生，骆清铭. 实时定量测量荧光共振能量转移效率的方法. 国家发明专利，2004，专利号：ZL 00114550.9
(3)陈同生，于怀娜. 一种FRET效率定量检测方法。国家发明专利，2014，专利号：ZL20111032899.8
(4) 陈同生， 王龙祥. 基于光漂白的荧光共振能量效率的定量测量方法. 国家发明专利2010, 专利号：ZL 200910039454.9
(5)陈同生，魏丽春，张江. 一种基于双通道荧光强度同时探测的荧光共振能量转移(FRET)效率(E)快速测量方法. 国家发明专利 2019, 专利号 ：ZL201611037454.1
(6)陈同生，杜孟艳. 一种基于激发光谱和发射光谱同时分离的FRET定量检测修正方法。国家发明专利 2019.03，授权专利号：ZL201710020313.7
(7)陈同生，张晨爽，林方睿。一种基于激发发射光谱分离同时测量受体-供体量子产额之比和消光系数之比的方法. 国家发明专利 2019.7, 授权专利号：ZL201710649949.8
(8)陈同生, 麦子昊. 一种基于供体-受体浓度比预处理FRET双杂交检测数据的方法. 国家发明专利，2020.04，授权专利号：ZL201810250537.1
(9)陈同生，孙晗，尹傲. 一种同时自动测量FRET系统矫正参数和供受体消光系数比的方法及其应用，授权专利号：ZL 202011012673.0 ，2022.03.15(有清远创新研究院)
(10) 陈同生，孙晗，庄正飞. 基于同一体系细胞样本测量激发发射光谱线性分离定量FRET系统校正因子的方法及应用，2021.03.13，授权专利号：ZL 202110270760.4
(11) 陈同生，刘曙通，孙晗，庄正飞. 一种自适应阈值的细胞图像分割方法，2021.8.23，申请号：202110970486.1,公开号：CN113658208A (有清远创新研究院)
(12) 陈同生，刘智. 基于一种标准FRET质粒测量FRET系统矫正参数的方法及其应用，授权专利号：ZL 202111083669.8
(13) 陈同生，高璐. 基于一次成像测量系统校正因子的QuanTi-FRET方法及其应用，授权专利号：ZL 202111211348.1
(14) 陈同生，王正印，王硕. 用于双通道FRET成像的相机安装装置及其校正方法，授权专利号：ZL 202210428918.0
(15) 陈同生，左欢，庄正飞，胡敏. 一种用于定量FRET显微成像的LED激发光源及调光方法，授权专利号：ZL 202210872007.7
(16) 陈同生，罗泽伟. 基于空间共定位掩模过滤的超分辨SIM-FRET图像重建方法，申请号：202210332681.6
(17) 庄正飞、叶梓轩、庄朝辉、陈同生。一种LED棒灯。专利申请号：202111295473.5（实质审查阶段）
(18) 陈同生，刘曙通。FRET自动测量系统（FRET Auto-Measurement System）。计算机软件著作权确认书，登记号：2021SR0720091， 证书号：软著登字第7442717号，2021年05月19日
(19) 陈同生 孙晗。Two Hybrid FRET Assay Software[简称：双杂交FRET分析软件]。计算机软件著作权确认书，登记号：2022SR47688，证书号：软著登字第9101887号，校内编号：2022007。2022年01月24日
(20) 梁振浩，陈同生，权婷婷。 一种用于检测甲醛的荧光探针及其制备方法和应用. 申请号：202110327401.8
(21) 梁振浩，蒋晓，陈同生. 一种用于检测硫化氢的荧光探针及其制备方法和应用. 申请号：202210342792.5
(22) 罗泽伟，毕晨阳，陈同生. 一种FRET敏化通道超分辨SIM图像重构参数的估计方法及系统. 申请号：202310321557.4
(23) 罗泽伟，孔梦婷, 陈同生. 一种用于定量FRET成像的激发光强度自动校准装置及方法. 申请号：202310235910.7
(24) 罗泽伟, 蔡竺祝, 陈同生. 基于TV-SIM重建的超分辨FRET成像方法、系统及介质. 申请号：202310355646.0
(25) 罗泽伟，李旭，王灵芝，陈同生. 基于自监督学习图像去噪的超分辨SIM-FRET成像方法与系统. 申请号：202310581280.9
(26) 胡敏，胡淏岚，陈同生. 基于荧光探针双通道亮度编码的快速多色成像方法. 申请号：202310369952.X
(27) 胡敏，王硕，陈同生. 一种用于双通道定量FRET显微成像的重影矫正方法. 申请号：202310819947.4
(28) 陈同生，王玥，胡敏. 一种FRET校准参数测量数据的预处理方法与系统. 申请号：202311495710.1
主要获奖
[1] 超分辨活细胞定量FRET成像分析仪，2023年第一届粤港澳大湾区博士博士后创新创业大赛创新赛金奖，罗泽伟，陈挚，王硕，曹庚强，武戈，陈鸿策，聂鹤羽（指导老师）
[2] 超分辨活细胞定量FRET成像分析仪，2023年第二届全国博士后创新创业大赛总决赛创新赛优胜奖，罗泽伟，陈挚，陈鸿策，万蓉，武戈，孙贝妮，聂鹤羽（指导老师）
[3] 智能定量FRET显微成像系统，2021年第一届全国博士博士后创新创业大赛创新赛金奖，陈同生，王正印，苏丽媚，汪昱彤，陈鸿策，孙晗，孙贝妮，罗泽伟。
[4] 组织光学成像理论若干关键问题研究，2003年度湖北省自然科学二等奖，骆清铭，曾绍群，朱丹，吴龟灵，陈同生。
[5] 光学与生命科学交叉科学中的前沿问题与关键技术研究.2009年度广东省科学技术奖（自然科学类）二等奖，邢达，朱德斌，陈同生，章春笋 张玲瑞，刘镭，唐永红等。
发表的主要论文清单（* 通讯作者）
2023
[1] Ge Wu, Fangfang Yang, Xuecheng Cheng, Zihao Mai, Xiaoping Wang*, Tongsheng Chen*. Live-cell imaging analysis on the anti-apoptotic function of the Bcl-xL transmembrane carboxyl terminal domain[J]. Biochemical and Biophysical Research Communications, 2023, 639: 91-99. DOI: 10.1016/j.bbrc.2022.11.104.
[2] Shutong Liu, Limei Su, Han Sun, Tongsheng Chen, Min Hu*, Zhengfei Zhuang*. Automated apoptosis identification in fluorescence imaging of nucleus based on histogram of oriented gradients of high-frequency wavelet coefficients[J]. Journal of Innovative Optical Health Sciences, 2023, 16(02): 2244003. DOI: 10.1142/S1793545822440035.
[3] Siqi He, Bo Xiao, Huajiang Wei, Shenjiao Huang*, Tongsheng Chen*. SVM classifier of cervical histopathology images based on texture and morphological features[J]. Technology and Health Care, 2023, 31(1): 69–80. DOI: 10.3233/THC-220031.
[4] Zihao Mai, Han Sun, Fangfang Yang, Mengyan Du, Xuecheng Cheng, Hongce Chen, Beini Sun, Wen Junlin, Xiaoping Wang*, Tongsheng Chen*. Bad is essential for Bcl-xL-enhanced Bax shuttling between mitochondria and cytosol[J]. The International Journal of Biochemistry & Cell Biology, 2023, 155: 106359. DOI: 10.1016/j.biocel.2022.106359.
[5] Zewei Luo, Ge Wu, Kong Mengting, Chen Zhi, Zhengfei Zhuang, Fan Junchao*, Tongsheng Chen*. Structured illumination-based super-resolution live-cell quantitative FRET imaging[J]. Photonics Research, 2023, 11(5): 887. DOI: 10.1364/PRJ.485521.
[6] Mo Yanquan, Wang Kunhao, Li Liujun, Xing Shijia, Ye Shouhua, Wen Jiayuan, Duan Xinxin, Luo Ziying, Gou Wen, Tongsheng Chen, Zhang Yuhui, Guo Changliang, Fan Junchao, Chen Liangyi. Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics[J]. Nature Communications, 2023, 14(1): 3089. DOI: 10.1038/s41467-023-38808-8.
[7] Zhang Yueyue, Han Weijing, Tongsheng Chen, Wang Shuang*. Mechanism of Sen1 translocation[J]. Acta Physica Sinica, 2023, 72(10): 108701. 张悦悦, 韩伟静, 陈同生, 王爽*.解旋酶Sen1行走机制研究[J]. 物理学报, 2023, 72(10): 108701. DOI: 10.7498/aps.72.20230187.
[8] Zhang Yuqiong, Han Weijing, Wang Lisha, Wang Hao, Jia Qi, Tongsheng Chen*, Wang Shuang*, Li Ming. Correlative Escherichia coli Transcription Rate and Bubble Conformation Remodeled by NusA and NusG[J]. The Journal of Physical Chemistry B, 2023, 127(13): 2909–2917. DOI: 10.1021/acs.jpcb.2c08771.
[9] Wang Zhaojun, Zhao Tianyu,Cai Yanan,Zhang Jingxiang, Hao Huiwen, Liang Yansheng, Wang Shaowei, Sun Yujie, Tongsheng Chen, Bianco Piero R., Oh Kwangsung, Lei Ming*. Rapid, artifact-reduced, image reconstruction for super-resolution structured illumination microscopy[J]. The Innovation, 2023, 4(3): 100425. DOI: 10.1016/j.xinn.2023.100425.
[10] Zhao Tianyu, Wang Zhaojun, Cai Yanan, Liang Yansheng, Wang Shaowei, Zhang Jingxiang, Tongsheng Chen, Lei Ming*. Fast single-layer reconstruction for three-dimensional structured illumination microscopy[J]. Optics and Lasers in Engineering, 2023, 167: 107606. DOI: 10.1016/j.optlaseng.2023.107606.
[11] Xing Shijia, Zheng Xiaolu, Yan Huifang, Mo Yanquan, Duan Ruoyu, Chen Zhixing, Wang Kunhao, Gao Kai, Tongsheng Chen, Zhao Shiqun, Wang Jinmin, Chen Liangyi*. Superresolution live-cell imaging reveals that the localization of TMEM106B to filopodia in oligodendrocytes is compromised by the hypomyelination-related D252N mutation[J]. Science China Life Sciences, 2023, 66(8): 1858-1868. DOI: 10.1007/s11427-022-2290-1.
[12] Miao Chen, Shuiling Lin, Xiaoyu Chen, Yu Wang, Jian Zhang, Tongsheng Chen*. An ultra-sensitive ESIPT fluorescent probe for distinguishing cancerous cells and diagnosing APAP-induced liver injury via HClO fluctuation[J]. Sensors and Actuators B: Chemical, 2023, 386: 133749. DOI: 10.1016/j.snb.2023.133749.
[13] Zhuo Zheng, Beini Sun, Siqi He, Guanchen Wang, Chenyang Bi, Tongsheng Chen*. Unsupervised deep learning of bright-field images for apoptotic cell classification[J]. Signal, Image and Video Processing, 2023, 17(7): 3657-3664. DOI: 10.1007/s11760-023-02592-1.
[14] Zewei Luo, Guodong Zang, Ge Wu, Mengting Kong, Zhengfei Zhuang, Tongsheng Chen*. High-fidelity SIM reconstruction-based super-resolution quantitative FRET imaging[J/OL]. Advanced Photonics Nexus, 2023, 2(05):056008 DOI: 10.1117/1.APN.2.5.056008.
[15] Shuo Wang , Min Hu*, Beini Sun, Tian Pan, Zhengfei Zhuang*, Tongsheng Chen. Dependence of ghost on the incident light angle into dichroic mirror[J]. Journal of Biophotonics, 2023, 16(11): e202300190 DOI: 10.1002/jbio.202300190.
[16] Zewei Luo, Ge Wu, Zhi Chen, Chinan Deng, Rong Wan, Tao Yang, Zhengfei Zhuang, Tongsheng Chen*. Dual-channel structured illumination super-resolution quantitative fluorescence resonance energy transfer imaging[J]. Acta Physica Sinica, 2023, 72(20): 208701. 罗泽伟, 武戈, 陈挚, 邓驰楠, 万蓉, 杨涛, 庄正飞, 陈同生*. 双通道结构光照明超分辨定量荧光共振能量转移成像系统[J]. 物理学报, 2023, 72(20): 208701. DOI: 10.7498/aps.72.20230853.
[17] Beini Sun, Hongce Chen, Xiaoping Wang*, Tongsheng Chen*. Regorafenib induces Bim-mediated intrinsic apoptosis by blocking AKT-mediated FOXO3a nuclear export[J]. Cell Death Discovery, 2023, 9(1): 37. DOI: 10.1038/s41420-023-01338-9.
[18] Xinzhuo Jiang, Min Hu*, Gengqiang Cao, Zhi Liu , Ge Wu, Zhengfei Zhuang, Tongsheng Chen*. Im-SCC-FRET: improved single-cell-based calibration of a FRET system[J]. Optics Express, 2023, 31(26): 43764. DOI: 10.1364/OE.503323.
[19] Jinqi Li, Xinlei Li*, Yangfeng Zhang, Kun Jin, Ye Yuan, Ruiqi Ming, Yuhua Yang, Tongsheng Chen*. An intravascular needle coated by ZnO nanoflowers for in vivo elimination of circulating tumor cells[J]. Nano Research, 2023, 16(1): 873–881. DOI: 10.1007/s12274-022-4764-x.
[20] Xuhong Fan, Yuting He, Guihao Wu, Hongce Chen, Xuecheng Cheng, Yongtong Zhan, Chunchun An, Tongsheng Chen, Xiaoping Wang* . Sirt3 activates autophagy to prevent DOX-induced senescence by inactivating PI3K/AKT/mTOR pathway in A549 cells[J]. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2023, 1870(2): 119411. DOI: 10.1016/j.bbamcr.2022.119411.
[21] Haozhe Li, Kai Zhang, Xiu Li, Baiquan Liu, Lianbi Li, Zengxia Mei, Tongsheng Chen, Qinzhuang Liu*, Wenzhi Yu*, Jian Yuan*, Haoran Mu*, Shenghuang Lin*. Two-dimensional (2D) α-In2Se3/Ta2NiSe5 heterojunction photodetector with high sensitivity and fast response in a wide spectral range[J]. Materials & Design, 2023, 227: 111799. DOI: 10.1016/j.matdes.2023.111799.
2022
[1] Zhi Liu, Zewei Luo, Hongce Chen, Ao Yin, Han Sun, Zhengfei Zhuang*, Tongsheng Chen*. Optical section structured illumination-based FRET imaging. Cytometry Part A, 2022, 101(3): 264-272. DOI: 10.1002/cyto.a.24500.
[2] Huanhuan Wang, Rumeng Qu, Qi Chen, Ting Zhang, Xiaoyu Chen, Baoyan Wu*, Tongsheng Chen*. PEGylated Prussian blue nanoparticles with attenuating tumor hypoxia and modulating polyethyleneimine cytotoxicity for dual-enhanced photodynamic therapy. Journal of Materials Chemistry B (accepted). DOI: 10.1039/D2TB00571A.
[3] Ruiqi Ming, Senyu Yang, Ye Jiang, Chunchun An, Yuhua Yang, Dongfeng Diao, Yuanyuan Cao, Tongsheng Chen*, and Xinlei Li*. Capture and isolation of tumor cells by graphene intercalated carbon film. Applied Physics Letters 2022, 120(6):063702. DOI: 10.1063/5.0082325.
[4] Ruiqi Ming， Ye Jiang, Jiaqi Fan, Chunchun An, Jinqi Li, Tongsheng Chen*, and Xinlei Li*. High-Efficiency Capture of Cells by Softening Cell Membrane. Small, 2022, 18(13): 2106547. DOI: 10.1002/smll.202106547
[5] Qiling Tang, Hongce chen, Zihao Mai, Han Sun, LingJun Xu, Guihao Wu, Zhuang Tu, Xuecheng Cheng, Xiaoping Wang* and Tongsheng Chen**. Bim- and Bax-mediated mitochondrial pathway dominates Abivertinib-induced apoptosis and ferroptosis. Free Radical Biology and Medicine, 2022(180): 198–209. DOI: 10.1016/j.freeradbiomed.2022.01.013.
[6] Guanling Zeng, Zhenhao Liang*, Xiao Jiang, Tingting Quan, Tongsheng Chen*. An ESIPT-Dependent AIE Fluorophore Based on HBT Derivative: Substituent Positional Impact on Aggregated Luminescence and its Application for Hydrogen Peroxide Detection. Chemistry A European Journal, 2022, 28(5): e202103241. DOI: 10.1002/chem.202103241
[7] Tingting Quan, Zhenhao Liang*, Huaiting Pang, Guanling Zeng, Tongsheng Chen*. A ratiometric ESIPT probe based on 2-aza-Cope rearrangement for rapid and selective detection of formaldehyde in living cells. Analyst, 2022, 147(2): 252-261. DOI: 10.1039/D1AN01722E.
[8] Miao Chen, Zhenhao Liang*, Guanling Zeng, Yu Wang, Zihao Mai, Xiaoyu Chen, Ge Wu, Tongsheng Chen*. An ESIPT-based NIR-emitting ratiometric fluorescent probe for monitoring hydrogen peroxide in living cells and zebrafish. Dyes and Pigments, 2022, 198: 109995. DOI: 10.1016/j.dyepig.2021.109995.
[9] Han Sun, Chenshuang Zhang, Ye Yuan, Lu Gao, Shixian Zhai, Hongce Chen, Qiling Tang, Zhengfei Zhuang *, Tongsheng Chen*. Automated ExEm-spFRET Microscope. Microscopy and Microanalysis, 2022, 28(2): 330-337. DOI: 10.1017/S1431927621013891.
[10] Limei Su, Shenjiao Huang*, Zhengyin Wang, Zhiqin Zhang, Huajiang Wei*, Tongsheng Chen*. Whole Slide Cervix Image Classification based on Convolutional Neural Network and Random Forest. International Journal of Imaging Systems and Technology, 2022, 32(3): 767-777. DOI: 10.1002/ima.22666.
[11] Pengfei Li, Yuan Bao, Boqian Chen, Xi Liu, Hui Yang, Quantong Li, Tongsheng Chen, Jianlin Fan, Chuan Hu, and Yunzhi Ling*. A Bioinspired Sweat-Drainable Janus Electrophysiological Electrode for Scientific Sports Training. Advanced Materials Technologies (accepted). DOI: 10.1002/admt.202200040.
2021年
[1] 陈同生，智能定量FRET显微成像系统，第一届全国博士后创新创业大赛金奖（2021年）
[2] 陈同生，全自动FRET成像分析仪开创者，2021广东省博士博士后创新创业大赛金奖
[3] Ting Lu，Senyu Yang, Bingqi Zhang, Xinlei Li*, and Tongsheng Chen*. Graphene oxide-doped photothermal heater in microchannel for thermophoretically shifting micro- and nano-particles[J]. Journal of Applied Physics, 2021, 130 (24): 244901. DOI: 10.1063/5.0073123.
[4] Hongce Chen, Beini Sun, Han Sun, Lingjun Xu, Guihao Wu, Zhuang Tu, Xuecheng Cheng, Xuhong Fan, Zihao Mai, Qiling Tang, Xiaoping Wang*, Tongsheng Chen**. Bak instead of Bax plays a key role in metformin-induced apoptosis in HCT116 cells. Cell Death Discovery, 2021, 7(1):363. DOI: 10.1038/s41420-021-00755-y.
[5] Han Sun, Ao Yin, Lu Gao, Hongce Chen, Qiling Tang, Ye Yuan,Zhi Liu, Zhengfei Zhuang*, Tongsheng Chen*. Measuring System Calibration Factors by Unmixing the Excitation–Emission Spectra of One Dish of Cells. Microscopy and Microanalysis, 2021, 27(6):1498-1505. DOI: 10.1017/S1431927621012642
[6] Ge Wu, Zhuang Tu, Fangfang Yang, Zihao Mai, Qiling Tang, Hongce Chen, Qiling Tang, Xianxin Ye, KunhaoWang, Xiaoping Wang*, Tongsheng Chen*. Evaluating the inhibitory priority of Bcl-xL to Bad, tBid and Bax by using live-cell imaging assay. Cytometry Part A, 2021, 99(11): 1091–1101. DOI: 10.1002/cyto.a.24351.
[7] Ao Yin, Han Sun, Hongce Chen, Zhi Liu, Qiling Tang, Ye Yuan, Zhuang Tu, Zhengfei Zhuang*, Tongsheng Chen*. Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids. CytometryPart A, 2021, 99(6): 632-640 . DOI: 10.1002/cyto.a.24316
[8] Lu Gao, Fangrui Lin, Danlu Han, Jieming Jiang, Chengwei Yang, Zhengfei Zhuang*, Tongsheng Chen*. Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts. Journal of Fluorescence, 2021, 31(4): 981-988. DOI: 10.1007/s10895-021-02728-x.
[9] Zechun Dang, Jizheng Sun, Jiaqi Fan, Jinqi Li, Xinlei Li*, and Tongsheng Chen*, Zinc oxide spiky nanoparticles: A promising nanomaterial for killing tumor cells, Materials Science & Engineering: C, 2021, 124: 112071. DOI: 10.1016/j.msec.2021.112071.
[10] Jiaqi Fan, Zechun Dang, Ting Lu, Jinqi Li, Tongsheng Chen*, Yuhua Yang, Xinlei Li*, Local release and isolation of circulating tumor cells captured by the nano-morphologic substrate coated with gelatin under near-infrared light. Journal of Materials Science, 2021, 56: 16634-16647. DOI: 10.1007/s10853-021-06364-5.
[11] 尹傲, 翟士贤, 孙晗, 刘智, 庄正飞*, 陈同生*. 活细胞定量FRET成像方法鲁棒性评估. 中国激光, 2021, 48(21): 2107001. DOI: 10.3788/CJL202148.2107001.
[12] 刘智, 罗泽伟, 王正印, 涂壮, 庄正飞*, 陈同生*. 基于结构照明的超分辨荧光显微成像重建算法. 中国激光, 2021, 48(3): 0307001. DOI: 10.3788/CJL202148.0307001.
2020年
[1] Mengyan Du, Si Yu, Wenhua Su, Mengxin Zhao, Fangfang Yang, Yangpei Liu, Zihao Mai, Yong Wang, Xiaoping Wang*, Tongsheng Chen*. Mitofusin 2 but not mitofusin 1 mediates Bcl-XL-induced mitochondrial aggregation. Journal of Cell Science 2020, 133(20): jcs245001. DOI doi: 10.1242/jcs.245001
[2] Fangfang Yang, Wenfeng Qu, Mengyan Du, Zihao Mai, Bin Wang, Yunyun Ma, Xiaoping Wang* & Tongsheng Chen*. Stoichiometry and regulation network of Bcl-2 family complexes quantified by live-cell FRET assay. Cellular and Molecular Life Sciences 2020, 77(12), 2387-2406. DOI: 10.1007/s00018-019-03286-z.
[3] Si Yu, Mengyan Du, Ao Yin, Zihao Mai, Yong Wang, Mengxin Zhao, Xiaoping Wang*, Tongsheng Chen*. Bcl-xL inhibits PINK1/Parkin-dependent mitophagy by preventing mitochondrial Parkin accumulation. International Journal of Biochemistry & Cell Biology 2020, 122: 105720. DOI: 10.1016/j.biocel.2020.105720.
[4] Yangpei Liu, Yunyun Ma, Zhuang Tu, Chenshuang Zhang, Mengyan Du,Yong Wang,, Fangfang Yang, Xiaoping Wang*, Tongsheng Chen*. Mcl-1 inhibits Mff-mediated mitochondrial fragmentation and apoptosis. Biochemical and Biophysical Research Communications, 2020, 523(3): 620-626. DOI: 10.1016/j.bbrc.2019.12.104.
[5] Yong Wang, Wenhua Su a, Zihao Mai, Si Yu, Xiaoping Wang*, Tongsheng Chen*. Anti-apoptotic capacity of Mcl-1D127. Biochemical and Biophysical Research Communications, 2020, 526(4): 1042-1048. DOI: 10.1016/j.bbrc.2020.03.181.
[6] Yong Wang, Wenhua Su, Zihao Mai, Mengyan Du, Si Yu, Yangpei Liu, Xiaopin Wang*, Tongshen Chen*. Co-expression of Mcl-1 and Bak induces mitochondrial swelling. Biochemical and Biophysical Research Communications 2020, 527(4): 866-873. DOI: 10.1016/j.bbrc.2020.04.154.
[7] 尹傲, 陈同生*. 基于光谱分离的定量荧光共振能量转移检测. 中国激光, 2020，47(2): 0207009.
[8] Fangfang Yang, Mengyan Du, Xiaoping Wang* and Tongsheng Chen*. Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging. Journal of Innovative Optical Health Sciences 2020, 13( 3): 2050011. DOI: 10.1142/S179354582050011X. （2020 IF 1.77）(Grant Nos.61527825, 61875056 and 81572184)
[9] Jing Zou, Jinqi Li, Tongsheng Chen and Xinlei Li*. Penetration mechanism of cells by vertical nanostructures, Physical Review E, 2020, 102(5): 052401. DOI: 10.1103/PhysRevE.102.052401.
[10] Jinqi Li, Jizheng Sun, Tongsheng Chen* and Xinlei Li*. Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis, Journal of Physics D: Applied Physics, 2020, 53(17): 175401. DOI: 10.1088/1361-6463/ab6fcc.
2019年
[1] Wenhua Su, Mengyan Du, Fangrui Lin, Chenshuang Zhang, Tongsheng Chen*. Quantitative FRET measurement based on spectral unmixing of donor, acceptor and spontaneous excitation-emission spectra. Journal of Biophotonics, 2019, 12(4): e201800314. DOI: 10.1002/jbio.201800314.
[2] Lu Wang, Zihao Mai, Mengxin Zhao, Bin Wang B, Si Yu, Xiaoping Wang*, Tongsheng Chen*. Aspirin induces oncosis in tumor cells. Apoptosis, 2019, 24(9-10):758–772. DOI: 10.1007/s10495-019-01555-7.
[3] Bin Wang, Zihao Mai, Mengyan Du, Lu Wang, Fangfang Yang, Yunyun Ma, Xiaoping Wang*, Tongsheng Chen*, BCL-XL directly retrotranslocates the monomeric BAK, Cellular signaling, 2019, 61: 1-9. DOI: 10.1016/j.cellsig.2019.05.001.
[4] Wenfeng Qu, Mengyan Du, Fangfang Yang, Zihao Mai, Chenshuang Zhang, Fangrui Lin, Yunyun Ma & Tongsheng Chen*. Gaussian FRET two-hybrid assays for determining the stoichiometry of hetero-oligomeric complexes in single living cells. Biochemical and Biophysical Research Communications, 2019, 512(3): 492-497. DOI: 10.1016/j.bbrc.2019.03.089.
[5] Wenfeng Qu, Zihao Mai, Chenshuang Zhang, Mengyan Du, Fangfang Yang & Tongsheng Chen*. Time-lapse FRET analysis reveals the ability of Bax dimer to trigger mitochondrial outer membrane permeabilization. Biochemical and Biophysical Research Communications, 2019, 514(3): 881-887. DOI: 10.1016/j.bbrc.2019.05.039.
[6] Chenshuang Zhang, Yangpei Liu, Wenfeng Qu, Wenhua Su, Mengyan Du, Fangfang Yang, Tongsheng Chen*. ExEm-FRET two-hybrid assay: FRET two-hybrid assay based on linear unmixing of excitation-emission spectra. Optics Express, 2019, 27(13): 18282. DOI: 10.1364/OE.27.018282.
[7] Han Sun, Chenshuang Zhang, Yunyun Ma, Mengyan Du, and Tongsheng Chen*. Controlling and online measurement of automatic dual-channel E-FRET microscope. Biomedical Signal Processing and Control, 2019, 53: 101585. DOI: 10.1016/j.bspc.2019.101585.
[8] Chenshuang Zhang, Yangpei Liu, Han Sun, Fangrui Lin, Yunyun Ma, Wenfeng Qu & Tongsheng Chen*. Automated E-FRET Microscope for Live-cell FRET Imaging. J. Microsc. 2019, 274(1): 45-54. DOI: 10.1111/jmi.12783.
[9] Jing Zhou, Yu Xiong, Zengchun Dang, Jinqi Li, Xinlei Li*, Yuhua Yang* and Tongsheng Chen*. Origin of efficiency enhancement in cell capture on nanostructured arrays. Journal of Materials Science, 2019, 54(5): 4236-4245. DOI: 10.1007/s10853-018-3108-4.
[10] Yunyun Ma, Mengyan Du, Fangfang Yang, Zihao Mai, Chenshuang Zhang, Wenfeng Qu, Bin Wang, Xiaoping Wang*, Tongsheng Chen*. Quantifying the inhibitory effect of Bcl-xl on the action of Mff using live-cell fluorescence imaging. FEBS Open Bio, 2019, 9(12): 2041–2051. DOI: 10.1002/2211-5463.12739.
[11] 孙晗，陈同生*. 用于在线E-FRET成像的背景自动识别与数据筛选. 光学学报. 2019, 2(39), 0217001.
[12] Junlin Wen, ZihaoMai, Mengxin Zhao, Xiaoping Wang*, and Tongsheng Chen. Full anti-apoptotic function of Bcl-XL complexed with Beclin-1 verified by live-cell FRET assays. Biochemical and Biophysical Research Communications, 2019, 511(3):700-704. DOI: 10.1016/j.bbrc.2019.02.107.
2018 年
[1] Fangrui Lin, Chenshuang Zhang, Mengyan Du, Lu Wang, Zihao Mai, Tongsheng Chen*. Superior robustness of ExEm-spFRET to IIem-spFRET method in live-cell FRET measurement. Journal of Microscopy, 2018, 272(2): 145–150. DOI: 10.1111/jmi.12755.
[2] Mengyan Du†, Fangfang Yang†, Zihao Mai, Wenfeng Qu, Fangrui Lin, Lichun Wei, and Tongsheng Chen*. FRET two-hybrid assay by linearly fitting FRET efficiency to concentration ratio between acceptor and donor. Applied Physics Letters, 2018, 112(15): 153702. DOI: 10.1063/1.5021466.
[3] Chenshuang Zhang, FangRui Lin, Mengyan Du, Wenfeng Qu, Zihao Mai, Junle Qu*, & Tongsheng Chen*. Simultaneous measurement of quantum yield ratio and extinction coefficient ratio between acceptor and donor by linearly unmixing excitation-emission spectra. Journal of Microscopy, 2018, 270(3): 335–342. DOI: 10.1111/jmi.12687.
[4] Mengyan Du, Zihao Mai, Fangrui Lin, Fangfang Yang, Lichun Wei, Tongsheng Chen*. Multi-channel wide-field microscopic FRET imaging based on simultaneous spectral unmixing of excitation and emission spectra. Journal of Microscopy, 2018, 269(1): 66–77. DOI: 10.1111/jmi.12609.
[5] Fangrui Lin, Mengyan Du, Fangfang Yang, Lichun Wei, and Tongsheng Chen*. Improved spectrometer-microscope for quantitative FRET measurement based on simultaneous spectral unmixing of excitation and emission spectra. Journal of Biomedical Optics, 2018, 23(1): 016006. DOI: 10.1117/1.JBO.23.1.016006.
[6] Jing Zhou, Xiaowei Zhang, Jizheng Sun, Zengchun Dang, Jinqi Li, Xinlei Li* and Tongsheng Chen*. The effects of surface topography of nanostructure arrays on cell adhesion. Physical Chemistry Chemical Physics, 2018, 20(35): 22946—22951. DOI: 10.1039/C8CP03538E.
2017年
[1] Lichun Wei, Jiang Zhang, Zihao Mai, Fangfang Yang, Mengyan Du, Fangrui Lin, Junle Qu*, Tongsheng Chen*. Quantitative dual-channel FRET microscopy. Optics Express, 2017, 25(21): 26089-26102. DOI: 10.1364/OE.25.026089.
[2] Liping Wu, Jinshan Xie, Tan Li, Zihao Mai, Lu Wang, Xiaoping Wang and Tongsheng Chen*. Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in eleven different cell lines. Royal Society Open Science, 2017, 4(10): 170822. DOI: 10.1098/rsos.170822.
[3] Yilin Pang, Zihao Mai, Bin Wang, Lu Wang, Liping Wu, Xiaoping Wang* and Tongsheng Chen*. Artesunate-modified nano-graphene oxide for chemophotothermal cancer therapy. Oncotarget, 2017, 8(55): 93800-93812. DOI: 10.18632/oncotarget.21191.
[4] Liping Wu, Yilin Pang, Guiqi Qin, Shengnan Wu, Xiaoping Wang*, Tongsheng Chen*. Farnesylthiosalicylic acid sensitizes hepatocarcinoma cells to artemisinin derivatives. Plos One, 2017, 12(2): e0171840. DOI: 10.1371/journal.pone.0171840.
[5] Ying-Yao Quan, Yu-Hong Liu1, Chun-Mei Lin, Xiao-Ping Wang*, Tong-Sheng Chen. Peroxynitrite dominates sodium nitroprusside-induced apoptosis in human hepatocellular carcinoma cells. Oncotarget, 2017, 8(18): 29833-29845. DOI: 10.18632/oncotarget.16164
[6] Chunmei Lin, Gaina Xi, Tan Li, Xiaoping Wang*, Tongsheng Chen. Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9. Applied Nanoscience, 2017, 7(8): 723–730. DOI: 10.1007/s13204-017-0613-8.
[7] Ying-yao Quan, Qiang Xia, Yu-hong Liu, Chun-mei Lin, Sheng-nan Wu, Xiao-ping Wang*, Tong-sheng Chen. Inhibitory effects of free and nano-liposomal-loaded resveratrol on sodium nitroprusside-induced rabbit chondrocyte apoptosis. Journal of Nanoscience and Nanotechnology, 2017, 17(3): 1740-1746. DOI: 10.1166/jnn.2017.13008.
2016年
[1] Tan Li, Liping Wu, Jiang Zhang, Gaina Xi, Yilin Pang, Xiaoping Wang and Tongsheng Chen*. Hydrothermal Reduction of Polyethylenimine and Polyethylene Glycol Dual-Functionalized NanoGraphene Oxide for high-efficiency Gene Delivery. ACS Applied Materials & Interfaces, 2016, 8(45): 31311−31320. DOI: 10.1021/acsami.6b09915.
[2] Tan Li, Hongyu Liu, Gaina Xi, Yiling Pang, Liping Wu, Xiaoping Wang *, Tongsheng Chen*. One step reduction and PEIylation of PEGylated nanographene oxide for high-efficient chemo-photothermal therapy. Journal of Materials Chemistry B, 2016, 4(17): 2972-2983. DOI: 10.1039/C6TB00486E.
[3] Yingyao Quan, Guiqi Qin, Hao Huang, Yuhong Liu, Xiaoping Wang, Tongsheng Chen*. Dominant roles of Fenton reaction in sodium nitroprusside-induced chondrocyte apoptosis. Free Radical Biology & Medicine, 2016, 94: 135-144. DOI: 10.1016/j.freeradbiomed.2016.02.026.
[4] Mengyan Du, Lili Zhang, Shusen Xie, Tongsheng Chen*. Wide-field microscopic FRET imaging using simultaneous spectral unmixing of excitation and emission spectra. Optics Express, 2016, 24(14): 16037-16051. DOI: 10.1364/OE.24.016037.
[5] Gaina Xi, Xiaoping Wang, Tongsheng Chen*. A reduced graphene oxide-based FRET sensor for highly sensitive detection of matrix metalloproteinase 2. International Journal of Nanomedicine, 2016, 11:1537–1547. DOI: 10.2147/IJN.S102517.
[6] Jiang Zhang, Fangrui Lin, Liuying Chai, Lichun Wei, Tongsheng Chen*. IIem-spFRET: Improved Iem-spFRET method for robust FRET measurement. Journal of Biomedical Optics, 2016, 21(10): 105003. DOI: 10.1117/1.jbo.21.10.105003.
[7] Yilin Pang, Guiqi Qin, Liping Wu, Xiaoping Wang, Tongsheng Chen*. Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells. Experimental Cell Research, 2016, 347(2): 251–260. DOI: 10.1016/j.yexcr.2016.06.012.
[8] Hongyu Liu, Tan Li, Yuhong Liu, Guiqi Qin, Xiaoping Wang, and Tongsheng Chen*. Glucose-reduced Graphene Oxide with Excellent Biocompatibility and Photothermal Efficiency as well as Drug Loading. Nanoscale Research Letters, 2016, 11(1): 211. DOI: 10.1186/s11671-016-1423-8.
[9] Jiang Zhang, Lili Zhang, Liuying Chai, Fangfang Yang, Mengyan Du, Tongsheng Chen*. Reliable measurement of the FRET sensitized-quenching transition factor for FRET quantification in living cells. Micron 2016, 88:7-15. DOI: 10.1016/j.micron.2016.04.005.
[10] ZP Wang, Y Li, Q Zhou, YF Wang, TS Chen. Berberine-loaded solid lipid nanoparticles enhance hypoglycemic efficacy on streptozotocin-induced diabetic C57BL/6 mice. Nanomed-Nanotechnol Biol Med 2016, 12(2): 483-484.
[11] Xinlei Li and Tongsheng Chen. Enhancement and suppression effects of a nanopatterned surface on bacterial adhesion. Physical Review E, 2016, 93(5): 052419. DOI: 10.1103/PhysRevE.93.052419.
[12] Hao Huang, Ying-yao Quan, Xiao-ping Wang, Tong-sheng Chen. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes. Nanoscale Research Letters, 2016, 11(1): 249. DOI: 10.1186/s11671-016-1461-2.
2015年
[1] Lili Zhang, Guiqi Qin, Liuying Chai, Jiang Zhang, Fangfang Yang, Hongqin Yang, Shusen Xie, Tongsheng Chen*. Spectral wide-field microscopic FRET imaging in live cells. Journal of Biomedical Optics, 2015, 20(8), 086011. DOI: 10.1117/1.JBO.20.8.086011.
[2] Guiqi Qin, Hongyu Liu, Liping Wu, Yilin Pang, Chubiao Zhao, Shengnan Wu, Xiaoping Wang, Tongsheng Chen*. Artesunate induces apoptosis via a ROS-independent Bax-mediated intrinsic pathway in HepG2 cells. Experimental Cell Research, 2015, 336(2):308-317. DOI: 10.1016/j.yexcr.2015.07.004.
[3] Guiqi Qin, Lili Zhang, Hongyu Liu, Yingyao Quan, Liuying Chai, Shengnan Wu, Xiaoping Wang, Tongsheng Chen*. Dihydroartemisinin induces apoptosis preferentially via a Bim/Bak-mediated intrinsic pathway in hepatocarcinoma cells. Apoptosis, 2015, 20: 1072–1086.
[4] Liuying Chai, Jianwei Zhang, Lili Zhang, Tongsheng Chen*. Miniature fiber optic spectrometer-based quantitative fluorescence resonance energy transfer measurement in single living cells," Journal of Biomedical Optics, 2015, 20(3): 037008. DOI: 10.1117/1.JBO.20.3.037008.
[5] Bingling Chen, Zhouyi Guo, Tongsheng Chen*. Maximum Entropy Method-based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor-Acceptor Distance Distribution: Theory and Simulation. IEEE Photonics Journal, 2015, 7(3): 1-10. DOI: 10.1109/JPHOT.2015.2417168.
[6] Jianwei Zhang, Huali Li, Liuying Chai, Lili Zhang, JunLe Qu, Tongsheng Chen*. Quantitative FRET measurement via unmixing emission spectra with independent cross excitation correction. Journal of Microscopy, 2015, 257(2): 104–116. DOI: 10.1111/jmi.12189.
[7] Jianwei Zhang, Fangfang Yang, Liuying Chai, Lili Zhang, Junle Qu, Tongsheng Chen*. Spectral measurement of acceptor-to-donor extinction coefficient ratio in living cells. Micron, 2015, 68: 98–106. DOI: 10.1016/j.micron.2014.09.007.
[8] Zhiping Wang, Junbiao Wu, Qun Zhou, Yifei Wang, and Tongsheng Chen. Berberine Nanosuspension Enhances Hypoglycemic Efficacy on Streptozotocin Induced Diabetic C57BL/6 Mice. Evidence-Based Complementary and Alternative Medicine 2015, 239749. DOI: 10.1371/journal.pone.0091611.
2014年
[1] 陈同生，于怀娜。一种FRET效率定量检测方法。国家发明专利 （2014）专利号：ZL20111032899.8
[2] Jingqin Chen, Hongyu Liu, Chubiao Zhao, Guiqi Qin, Gaina Xi, Tan Li, Xiaoping Wang, and Tongsheng Chen*. One-step Reduction and PEGylation of Graphene Oxide for Photothermally Controlled Drug Delivery. Biomaterials, 2014, 35(18): 4986-4995. DOI: 10.1016/j.biomaterials.2014.02.032.
[3] Lili Zhang, Huaina Yu, Jianwei Zhang, Xiaoping Wang, Tongsheng Chen*. Binomial distribution-based quantitative measurement of multiple-acceptors fluorescence resonance energy transfer by partially photobleaching acceptor. Applied Physics Letters, 2014, 104(24): 243706. DOI: 10.1063/1.4885084.
[4] Chubiao Zhao, Guiqi Qin, Weijie Gao, Jingqin Chen, Hongyu Liu, Gaina Xi, Tan Li, Shengnan Wu, Tongsheng Chen*. Potent proapoptotic actions of dihydroartemisinin in gemcitabine-resistant A549 cells. Cellular Signalling, 2014, 26(10): 2223–2233. DOI: 10.1016/j.cellsig.2014.07.001.
[5] Chubiao Zhao, Weijie Gao, Tongsheng Chen*. Synergistic induction of apoptosis in A549 cells by dihydroartemisinin and gemcitabine. Apoptosis, 2014, 19(4): 668–681. DOI: 10.1007/s10495-013-0953-0.
[6] Jingqing Chen, Xiaoping Wang, Tongsheng Chen*. Facile and green reduction of covalently PEGylated nano-graphene oxide via a “water-only” route for high-efficiency photothermal therapy. Nanoscale Research Letters, 2014, 9(1):86. DOI: 10.1186/1556-276X-9-86.
[7] Qian Liang, Xiao-ping Wang, Tong-sheng Chen. Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS. Apoptosis, 2014, 19(9): 1354-1363. DOI: 10.1007/s10495-014-1012-1.
[8] Hua Jin, Qian Liang, Tongsheng Chen, XiaoPing Wang. Resveratrol protects chondrocytes from apoptosis via altering the ultrastructural and biomechanical properties: an AFM study. PLOS ONE, 2014, 9(3): e91611. DOI: 10.1371/journal.pone.0091611.
2013年
[1] Weijie Gao, Fenglian Xiao, Xiaoping Wang, Tongsheng Chen*. Artemisinin induces A549 cell apoptosis dominantly via a reactive oxygen species-mediated amplification activation loop among caspase-9, -8 and -3. Apoptosis, 2013, 18(10): 1201-1213. DOI: 10.1007/s10495-013-0857-z.
[2] Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang, Tongsheng Chen*, Junle Qu,* and Hanben Niu. Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector. Optical Letter, 2013, 38(10):1697-1699. DOI: 10.1364/OL.38.001697.
[3] Tongsheng Chen*, Min Chen, Jingqin Chen. Dihydroartemisinin induces apoptosis and sensitizes cells to low-dose ionizing radiation largely by a ROS-mediated extrinsic apoptosis pathway in A549 cells. Plos One, 2013, 8(3): e59827. DOI: 10.1371/journal.pone.0059827.
[4] Huaina Yu, Jianwei Zhang, Huali Li, and Tongsheng Chen*. Ma-PbFRET: Multiple Acceptors FRET Measurement Based on Partial Acceptor Photobleaching. Microscopy and Microanalysis, 2013, 19(1): 171-179. DOI: 10.1017/S1431927612014079.
[5] Caiping Zhuang, Xiaoping Wang, and Tongsheng Chen*. H2o2 Induces Apoptosis Of Rabbit Chondrocytes Via Both The Extrinsic and The Caspase-Independent Intrinsic Pathways. Journal of Innovative Optical Health Sciences, 2013, 6(3): 1350022. DOI: 10.1142/S1793545813500223.
[6] YingYao Quan, Chaoyang Wang, XiaoPing Wang, Tongsheng Chen. Taxol induces cell death with cytoplasm vacuolization in paraptosis-like but not oncosis fashion in ASTC-a-1 cells. Journal of Innovative Optical Health Sciences, 2013, 6(4): 1350046. DOI: 10.1142/S1793545813500466.
2012年
[1] Huali Li, Huaina Yu, Tongsheng Chen*. Quantitative measurement of FRET efficiency free from spectral bleed-through in living cells. Microscopy and Microanalysis, 2012, 18(5): 1021–1029. DOI: 10.1017/S1431927612001110.
[2] Huaina Yu, Jianwei Zhang, Huali, Li Junle Qu, Tongsheng Chen*. An empirical quantitative fluorescence resonance energy transfer method for multiple acceptors based on partial acceptor photobleaching. Applied Physics Letters, 2012, 100(25): 253701. DOI: 10.1063/1.4729481.
[3] WeiWei Zhang, XiaoPing Wang, Tongsheng Chen*. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells. Cellular Signaling, 2012, 24(5):1037-1046. DOI: 10.1016/j.cellsig.2011.12.025.
[4] Chenjun Zhou, Wenliang Pan, Tongsheng Chen*. Artesunate induces apoptosis largely via a Bak-mediated caspases-independent intrinsic pathway in human lung adenocarcinoma cells. Journal of Cellular Physiology, 2012, 227(12):3778-3786. DOI: 10.1002/jcp.24086.
[5] Fenglian Xiao, Weijie Gao, Xiaoping Wang, Tongsheng Chen*. Amplification activation loop between caspase-8 and -9 dominates artemisinin-induced apoptosis of ASTC-a-1 cells. Apoptosis, 2012, 17(6): 600-611. DOI: 10.1007/s10495-012-0706-5.
[6] Chaoyang Wang, Tongsheng Chen*. Intratumoral injection of taxol in vivo suppresses A549 tumor showing cytoplasmic vacuolization. Journal of Cellular Biochemistry, 2012, 113(4): 1397–1406. DOI: 10.1002/jcb.24012.
[7] Min Chen, Tong-sheng Chen*, Ying-ying Lu, Cheng-yi Liu, Jun-le Qu. Dihydroarteminsin (DHA) induced apoptosis is not dependent on the translocation of Bim to the endoplasmic reticulum in human lung adenocarcinoma cells. Pathology & Oncology Research, 2012, 18(4): 809-816. DOI: 10.1007/s12253-012-9508-x.
[8] Haiyang Li, Xiaoping Wang, Tongsheng Chen*. P38 Inhibitor SB203580 Sensitizes the Resveratrol-Induced Apoptosis in Human Lung Adenocarcinoma (A549) Cells. Journal of Biochemical and Molecular Toxicology, 2012, 26(7): 251-257. DOI: 10.1002/jbt.21413.
[9] XiaoPing Wang，JianWei Zhang, Huai-na Yu, Tong-sheng Chen*. Quantitatively monitoring the caspase-3 activation in single live cells using partial acceptor photobleaching FRET method. Journal of Innovative Optical Health Sciences, 2012，5(3), 1250015. DOI: 10.1142/S1793545812500150.
[10] 陈同生，于怀娜.一种基于受体部分光漂白和供体-受体交替激发的FRET效率定量检测方法. 国家发明专利，专利申请号：20110238171.4
2011年
[1] Lei Sun, Tongsheng Chen*, Xiaoping Wang, Yun Chen, Xunbin Wei. Bufalin Induces Reactive Oxygen Species (ROS)-Dependent Bax Translocation and apoptosis in ASTC-a-1 Cells. Evidence-Based Complementary and Alternative Medicinem, 2011, 2011: 249090. DOI: 10.1093/ecam/nep082.
[2] Wenjing Guo, Lei Qian, Jing Zhang, Wei Zhang, Tongsheng Chen*, and Jie Zhao. SIRT1 overexpression in neurons promotes neurite outgrowth and cell survival with reduced mTOR signaling. Journal of Neuroscience Research, 2011, 89(11): 1723-1736. DOI: 10.1002/jnr.22725.
[3] Weiwei Zhang, Xiaoping Wang, Tongsheng Chen*. Resveratrol induces mitochondria-mediated AIF- and to a lesser extent caspase-9-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Molecular and Cellular Biochemistry, 2011, 354(1-2): 29–37. DOI: 10.1007/s11010-011-0802-9.
[4] WeiWei Zhang, Zhi-Ping Wang, Tongsheng Chen*. Curcumol induces apoptosis via caspases-independent mitochondrial pathway in huaman lung adenocarcinoma ASTC-a-1 cells. Medical Oncology, 2011, 28(1):307-314. DOI: 10.1007/s12032-010-9431-5.
[5] Fenglian Xiao, Tongsheng Chen*, Chengyi Liu, Xiaoping Wang. Artemisinin induces caspase-8/9-mediated and Bax/Bak-independent apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. Journal of X-Ray Science and Technology, 2011，19(4): 545-555. DOI: 10.3233/XST-2011-0313.
2010年
[1] Li-hui Wang, Jin Xiang, Min Yan, Yan Zhang, Yan Zhao, Cai-feng Yue, Jie Xu, Fei-meng Zheng, Jin-na Chen, Zhuang Kang, Tong-sheng Chen, Da Xing, and Quentin Liu. Mitotic kinase Aurora-A induces mammary cell migration and breast cancer metastasis through up-regulating cofilin activity. Cancer Research, 2010， 70(22): 9118-9128. DOI: 10.1158/0008-5472.CAN-10-1246.
[2] 陈同生, 王龙祥. 基于光漂白的荧光共振能量效率的定量测量方法. 国家发明专利, 专利号: ZL200910039454.9.
[3] Qingrui Sun, Tong Sheng Chen*, Xiao Ping Wang, Xunbin Wei. Taxol induces paraptosis independent of protein synthesis and MAPK pathway. Journal of Cellular Physiology, 2010, 222(2): 421-432. DOI: 10.1002/jcp.21982.
[4] Ying-Ying Lu, Tong-Sheng Chen*, Xiao-Ping Wang, Jun-Le Qu. The JNK inhibitor SP600125 enhances dihydroartemisinin-induced apoptosis by accelerating Bax translocation into mitochondria in human lung adenocarcinoma cells. FEBS Letter, 2010, 584(18): 4019–4026. DOI: 10.1016/j.febslet.2010.08.014.（SCI IF 3.6）
[5] Ying-Ying Lu, Tong-Sheng Chen*, Xiao-Ping Wang , Li Li. Single cell analysis of dihydroartemisinin(DHA)-induced apoptosis through reactive oxygen species (ROS)-mediated caspase-8 activation and mitochondrial pathway in ASTC-a-1 cells using fluorescence imaging techniques. Journal of Biomedical Optics, 2010, 15(4): 046028. DOI: 10.1117/1.3481141.
[6] Min Chen, Ying-ying Lu, Tong-sheng Chen*，Jun-le Qu. Confocal Imaging of Bim Translocation to ER during DHA-Induced ASTC-a-1 Cell Apoptosis. Chinese Optical Letter, 2010, 8(10):950-953. DOI: 10.3788/COL20100810.0950.
[7] Huai-na Yu, Tong-sheng Chen*, Jun-le Q. Improving FRET efficiency measurement in confocal microscopy imaging. Chinese Optical Letter, 2010, 8(10): 947-949. DOI: 10.3788/COL20100810.0947.
[8] Longxiang Wang, Tongsheng Chen*, Junle Qu, Xunbin Wei. Photobleaching-Based Quantitative Analysis of Fluorescence Resonance Energy Transfer Inside Single Living Cell. Journal of Fluorescence, 2010, 20(1):27-35. DOI: 10.1007/s10895-009-0518-3.
[9] Wenjing Guo, Tongsheng Chen*, Xiaoping Wang, Rong Chen. Taxol induces concentration-dependent cell death form in ASTC-a-1 cells. Journal of X-Ray Science and Technology, 2010, 18(3): 293–308. DOI: 10.3233/XST-2010-0261.
2009年
[1] Longxiang Wang，Tongsheng Chen*, JunLe Qu et al. Quantitative analysis of caspase-3 activation by fitting fluorescence emission spectra in living cells. Micron 2009, 40(12): 811–820. DOI: 10.1016/j.micron.2009.07.001.
[2] Ying-Ying Lu, Tong-Sheng Chen*, Jun-Le Qu, Wen-Liang Pan, Lei Sun, Xunbin Wei. Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma cells. Journal of Biomedical Science, 2009, 16(1): 16. DOI: 10.1186/1423-0127-16-16.
[3] Wenliang Pan, Junle Qu, Tongsheng Chen*, Lei Sun, Jing Qi. FLIM and emission spectral analysis of caspase-3 activation inside single living cell during anticancer drug-induced cell death. European Biophysics Journal, 2009, 38(4): 447–456. DOI: 10.1007/s00249-008-0390-0.
[4] Longxiang Wang, Xiangbo Yang, Tongsheng Chen. Second harmonic generation in generalized Thue–Morse ferroelectric superlattices. Physica B: Condensed Matter, 2009, 404(20): 3425-3430. DOI: 10.1016/j.physb.2009.05.027.
[5] Xiaoping Wang, Dongmei He, Jiye Cai, Tongsheng Chen, Feiyan Zou, Yalan Li ,Yangzhe Wu, Zheng W. Chen, Yong Chen. WGA-QD probe-based AFM detects WGA-binding sites on cell surface and WGA-induced rigidity alternation. Biochemical and Biophysical Research Communications, 2009, 379(2): 335–340. DOI: 10.1016/j.bbrc.2008.12.058.
2008年：
[1] Tong-sheng Chen*, Xiao-ping Wang, Lei Sun, Long-xiang Wang, Da Xing, Martin Mok. Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cell. Cancer Letters, 2008, 270(1): 164-172. DOI: 10.1016/j.canlet.2008.05.008.
[2] Lei Liu, Da Xing, Wei R. Chen, Tongsheng Chen, Yihui Pei, and Xuejuan Gao．Calpain-mediated pathway dominates cisplatin-induced apoptosis as determined by real-time single cell analysis. International Journal of Cancer, 2008, 122(10): 2210-2222. DOI: 10.1002/ijc.23378.
[3] Xiao-Ping Wang, Tong-Sheng Chen*, Lei Sun, Ji-Ye Cai, Ming-Qian Wu, Martin Mok．Live Morphological Analysis of Taxol-Induced Cytoplasmic Vacuoliazation in human lung adenocarcinoma cells. Micron 2008, 39(8): 1216-1221. DOI: 10.1016/j.micron.2008.04.007.
[4] Lei Sun, Xiao-Ping Wang, Tong-Sheng Chen*． Involvement of Bax and caspase-3 activation in Chan-Su-induced ASTC-a-1 cell apoptosis. Journal of X-Ray Science and Technology, 2008, 16(2): 131–142. DOI: 10.3233/XST-2008-0197.
[5] Tong-sheng CHEN*, Xiao-ping WANG, Lei Sun, Hui-ying WANG, Long-xiang WANG．Caspase-3 is Involved in the Chan-Su(CS)-induced Human Lung Adenocarcinoma (ASTC-a-1) Cell Apoptosis. Progress in Biochemistry and Biophysics, 2008, 35(1): 83-90．
[6] Tong-sheng CHEN*, Xiao-ping WANG, Zhi-ping WANG, Long-xiang WANG, Hui-ying WANG. Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) Inside Living Human Lung Adenocarcinoma Cells. Spec & Spec Anal., 2008, 28(6): 1327-1331．
[7] Tong-sheng CHEN*, Xiao-ping WANG, Lei SUN, Hui-ying WANG, Long-xiang WANG. Fluorescence Analysis of Taxol-Induced Paraptosis-Like Independent of Caspase-3 Activation. Spec & Spec Anal, 2008, 28(11): 2623-2627．
[8] Xiao-ping Wang, Tongsheng Chen*, Longxiang Wang, Lei Sun. Live Imaging of Xiao-Ai-Ping-induced Cell Death in Human Lung Adenocarcinoma Cells. Journal of Innovative Optical Health Sciences, 2008, 1(1): 151-156. DOI: 10.1142/S1793545808000133.
2007年之前
[1] Tongsheng Chen, Jinjun Wang, Da Xing and Wei R. Chen. Spatio-temporal dynamic analysis of Bid activation and apoptosis induced by alkaline condition in human lung adenocarcinoma cell. Cellular Physiology and Biochemistry, 2007, 20(5): 569-578. DOI: 10.1159/000107540.
[2] Yihui Pei, Da Xing, Xuejuan Gao, Tongsheng Chen, Lei Liu. Real-time Monitoring Full length Bid Interacting with Bax during TNF-α-induced Apoptosis. Apoptosis, 2007, 12(9): 1681-1690. DOI:10.1007/s10495-007-0091-7.
[3] Yin-Yuan WU, Da XING, Lei LIU, Tong-sheng CHEN, and Wei CHEN. Fluorescence Resonance Energy Transfer Analysis of Bid Activation in Living Cells during Ultraviolet-induced Apoptosis. Acta Biochimica et Biophysica Sinica, 2007, 39(1): 37-45. DOI: 10.1111/j.1745-7270.2007.00246.x.
[4] Miaojuan Chen, Da Xing, Tongsheng Chen, and Lan Zhang. BimL involvement in Bax activation during UV irradiation-induced apoptosis. Biochemical and Biophysical Research Communications, 2007, 358(2), 559-565. DOI: 10.1016/j.bbrc.2007.04.167.
[5] Shengnan Wu, Da Xing, Fang Wang, Tongsheng Chen, Wei R. Chen, Mechanistic Study of Apoptosis Induced by High Fluence Low Power Laser Irradiation Using Fluorescence Imaging Techniques. Journal of Biomedical Optics, 2007, 12(6): 064015. DOI: 10.1117/1.2804923.
[6] Xuejuan Gao#, Tongsheng Chen#, et al. Single Cell Analysis of PKC Activation During Proliferation and Apoptosis Induced by Laser Irradiation. Journal of Cellular Physiology, 2006, 206(2): 441-448. DOI: 10.1002/jcp.20484. (# 并列第一作者)
[7] Fang Wang#, Tong-Sheng Chen#, et al. Measuring Dynamics of Caspase-3 Activity in Living Cells Using FRET Technique During Apoptosis Induced by High Fluence Low Power Laser Irradiation. Laser in Surgery and Medicine, 2005, 36(1): 2-7. DOI: 10.1002/lsm.20130.
[8] Fang WANG, Tong-Sheng CHEN, Da XING. Measuring dynamics of caspase-3 activation in living cell by FRET during high dose low energy laser irradiation-induced cell apoptosis. SPIE, 2005, 5630: 593.
[9] 陈同生，曾绍群，骆清铭. 基于调Q脉冲激光的多光子激发荧光方法. 国家发明专利，2004，专利号：ZL 01106653.9.
[10] 曾绍群，陈同生，骆清铭. 实时定量测量荧光共振能量转移效率的方法. 国家发明专利，2004，专利号：ZL OO114550.9.
[11] TongSheng Chen， Shaoqun Zeng, Qingming Luo et al., A Quantitative Theory Model of Photobleaching Mechanism. Chinese Physics Letter, 2003, 20(11): 1940-1943.
[12] 陈同生,曾绍群，骆清铭 等，供体受体双光子激发吸收截面比值的测量, 自然科学进展, 2003, 13(12): 1312-1315.
[13] TongSheng Chen，Shaoqun Zeng, Qingming Luo et.al. High-Order Photobleaching of Green Fluorescent Protein inside live Cells in Two-Photon Excitation Microscopy. Biochemical and biophysical research communications, 2002, 291(5): 1272-1275
会议论文：(* 通讯作者)
1 Hai-yang Li, Cai-ping Zhuang, Xiao-ping Wang, Tong-sheng Chen*. SB203580 enhances the RV-induced loss of mitochondrial membrane potential and apoptosis in A549 cells. Proc. of SPIE 2012, 8224, 82240L(EI收录)
2 Chao-yang Wang, Tong-sheng Chen*.Taxol-induced paraptosis-like A549 cell death is not senescence. Proc. of SPIE 2011, 7900: 79000K-1 （EI 收录）
3 Huai-na Yu, Ying-ying Lu, Tong-sheng Chen*. Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Proc. of SPIE 2011, 7900: 79000J-1 （EI 收录）
4 Wen-jing Guo, Tong-sheng Chen*. Taxol induces concentration-dependent phosphatidylserine (PS) externalization and cell cycle arrest in ASTC-a-1 cells. Proceedings of the SPIE, 7565：75650J-75650J-7 (2010). （EI 收录）
5 Feng-lian Xiao, Tong-sheng Chen*, Jun-Le Qu, Cheng-Yi Liu. Artemisinin induces ROS-mediated caspase3 activation in ASTC-a-1 cells. Proceedings of the SPIE, Volume 7565, pp. 75650I-75650I-7 (2010)， (EI 收录）
6 Wei-wei Zhang, Zhi-ping Wang, Tong-sheng Chen*. Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells. Proceedings of the SPIE, Volume 7565, pp. 75650K-75650K-7 (2010)， (EI 收录）
7 Ying-Ying Lu, Tong-Sheng Chen*，Jun-Le Qu. Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells. Proc. of SPIE 2009， 7178, 71780J
8 Wen-liang Pan, Tong-sheng Chen*, Jun-Le Qu. Emission spectral analysis of caspase-3 activation during artesunate-induced apoptosis of human lung adenocarcinoma cell. Proc. of SPIE 2009， 7178, 71780I
9 Qingrui Sun, Tongsheng Chen*. Reactive oxygen species (ROS) is not a promotor of taxol-induced cytoplasmic vacuolization. Proc. of SPIE 2009，7178, 71780K
10 Tong-sheng Chen *, Lei Sun, Huiying Wang, Longxiang Wang．Fluorescence Imaging Analysis of Taxol-Induced Cell Death with Cell Swelling and Cytoplasmic Vacuolization．Proceedings of the SPIE 2008, 6857, 68570L-68570L-8 (2008) (EI 收录)．
11 Lei Sun, Tongsheng Chen*, Longxiang Wang, Huiying Wang．Analysis of caspase3 activation in ChanSu-induced Apoptosis of ASTC-a-1 Cells by Fluorescence Techniques．Proceedings of the SPIE 2008, 6857, 68570I-68570I-8 (2008) (EI 收录)．
12 Huiying Wang, Tongsheng Chen*, Lei Sun．Analysis of caspase-3 activation in mitomycin C-induced ASTC-a-1 cell apoptosis using acceptor photobleaching techniques．Proceedings of the SPIE 2008, 6857, pp. 68570J-68570J-7 (2008) (EI 收录)．
13 Tongsheng Chen, et al. Dynamic imaging of interaction between proteins 14-3-3 and Bid in living cells, SPIE, 2006, 6087:60870L (EI 收录)
14 Tongsheng Chen, et al. Real-time single cell analysis of molecular mechanism of apoptosis and preliforaion using FRET technique, SPIE, 2006, 6047:604736 (EI 收录)
15 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2005, 5630: 368（EI收录）
16 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2004, 5630:368 (EI 收录)
17 Tongsheng Chen, Shaoqun Zeng, Qingming Luo et al., High-Order Photobleaching in Two-Photon Excitation FluorescenceMicroscopy Inside Live Cell. SPIE. 2003 . 4967: 63-67（EI收录）
18 Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, Qingming Luo. Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy. SPIE, 2003，5254:508-512（EI收录）
19 Tongsheng Chen, Shaoqun Zeng, Qingming Luo. Interaction way of photobleaching produced bythe interaction between excitation photons and fluorophores molecules in excited states. SPIE,2003，4967:185-190（EI收录）
20 Tongsheng Chen, et. al. Data analysis in green fluorescent protein based resonance energy transfer. SPIE. 2000, 4224: 346-350 (EI 收录)。